Diagnose chromatographic symptoms systematically.
SEPAX Technologies offers chromatography columns engineered for reliable analytical and preparative separations across pharmaceutical, biopharmaceutical, biotechnology, food, environmental and research laboratories. This page provides technical guidance, applications and specifications to help select the appropriate SEPAX column.
High backpressure
Check instrument pressure without the column, inspect inlet frit contamination, verify mobile-phase miscibility, remove precipitates and confirm flow, viscosity and temperature.
Peak tailing
Evaluate active interactions, pH and buffer strength, sample overload, extra-column connections, contamination and column condition.
Peak splitting
Check injection solvent strength, inlet voids, blocked frits, poor connections, sample insolubility and method equilibration.
Low resolution
Review selectivity first, then retention, efficiency, gradient slope, temperature, flow and column dimensions.
Broad peaks
Check extra-column volume, flow, loading, diffusion, sample solvent, detector settings and column efficiency.
Retention drift
Confirm mobile-phase composition, pH, temperature, mixing accuracy, equilibration, column history and sample matrix.
Ghost peaks
Run blanks, isolate solvent and instrument sources, review carryover, mobile-phase purity, injector wash and column contamination.
Column washing
Use a product-compatible sequence that removes buffer before stronger organic or alternative wash solvents. Avoid precipitation.
Storage
Follow product-specific instructions, remove incompatible salts and store in a suitable solvent with sealed end fittings.
Lifetime extension
Filter samples and mobile phases, use guards where appropriate, avoid pressure shocks and incompatible conditions, and document column history.
Preventive maintenance
Inspect fittings, guard devices, solvent filters, seals and injector wash performance; replace contaminated consumables before column damage occurs.
Need assistance?
Send the catalog number, method conditions, pressure history and chromatogram to Akira Analytical Solutions Pvt. Ltd..