Affinity Columns
SEPAX Affinity Columns support affinity chromatography for target biomolecules that interact selectively with an immobilized ligand or binding chemistry. Use the searchable catalog table below to compare the listed phases, particle sizes, pore sizes, dimensions and catalog numbers, then confirm the final configuration against the intended method and instrument requirements.
Affinity Columns specifications
| Product phase | Particle size (µm) | Dimensions | Catalog number |
|---|---|---|---|
| PSDVB-SBTI affinity column | 2.1 × 50 mm | 010091P-2105 | |
| ProAqa Excel | 4.6 × 50 mm | 271120980-4605P | |
| ProAqa Excel | 4.6 × 50 mm | 271120980-4605S | |
| ProAqa Excel | 4.6 × 35 mm | 271120980-4603S | |
| ProAqa Excel | 4.6 × 100 mm | 271120980-4610S | |
| ProAqa Excel | 4.6 × 100 mm | 271120980-4610P | |
| ProAqa Excel | 20 | 2.1 × 30 mm | 271120980-2103S |
| ProAqa Excel | 20 | 2.1 × 30 mm | 271120980-2103P |
| ProAqa Excel | 2.1 × 50 mm | 271120980-2105S | |
| ProAqa Excel | 2.1 × 50 mm | 271120980-2105P | |
| Affinity HSA | 5 | 4.6 × 50 mm | 272101P-4605 |
| Affinity HSA | 5 | 2.1 × 50 mm | 272101P-2105 |
| Desalting RP | 5 | 2.1 × 10 mm | 010072-2101 |
Overview
Named phases: Affinity HSA, Desalting RP, PSDVB-SBTI affinity column, ProAqa Excel
Separation categories: Affinity
Available particle sizes: 5, 20 µm
Scientific principle
Affinity chromatography uses selective interactions between a target and an immobilized ligand or binding chemistry.
For detailed surface chemistry and packing guidance, contact technical support.
What are SEPAX Affinity Columns?
SEPAX Affinity Columns are catalog-listed products for affinity chromatography. The family is intended for target biomolecules that interact selectively with an immobilized ligand or binding chemistry, with available configurations shown in the specification table on this page.
How do Affinity Columns work?
The separation is governed by selective and reversible target–ligand interaction. Selectivity also depends on the exact phase, mobile phase, sample properties, temperature, flow rate and column dimensions.
How to select a configuration
Start with the required separation mechanism and named phase. Then compare particle size, pore size where listed, internal diameter, length and catalog number. Match the configuration to system pressure capability, sample load and the scale of the method.
Applications
This family is relevant to targeted capture, cleanup, purification and analytical workflows. Suitability must be confirmed experimentally with the actual sample and method conditions.
Method development
Begin with a documented method or a chemically reasonable starting condition for the selected phase. Control sample solvent strength, buffer compatibility, pH, ionic strength, gradient profile, temperature and injection volume. Change one variable at a time and record system-suitability results.
Column care and storage
Filter samples and mobile phases, prevent buffer precipitation, avoid sudden solvent incompatibility and stay within the product-specific operating limits supplied with the column. After use, remove strongly retained material with a compatible wash and store the column only in a suitable solvent for that exact phase.
Replacement and method transfer
Do not select a replacement by dimensions alone. Compare separation mechanism, stationary-phase type, particle size, pore size where applicable, column geometry and method conditions. Verify equivalence through retention, resolution, efficiency, tailing and system-suitability testing.
For catalog identification and application support, contact SEPAX technical support in India.
Troubleshooting guidance
Loss of capacity can reflect ligand fouling, incomplete regeneration, sample aggregation, unsuitable loading conditions or repeated exposure to harsh cleaning agents.
Cleaning and regeneration
Regenerate only with solutions validated for the ligand and support. Remove residual sample promptly and store under conditions that protect biological activity and prevent microbial growth.
Mobile phase recommendations
Loading, washing and elution buffers must preserve ligand activity while controlling target binding. Optimize pH, ionic strength and additives carefully, and neutralize collected fractions promptly when low-pH elution is used.
Related SEPAX product families
For complementary selectivity or workflow steps, also review Proteomix Columns, Antibodix Columns and SEC Columns. These related families can support orthogonal method development, sample cleanup, confirmation or scale-up depending on the application.
Frequently asked questions
What are Affinity Columns?
Affinity Columns are SEPAX catalog products used for affinity chromatography. The listed configurations can be compared by phase, particle size, pore size where applicable, dimensions and catalog number.
How do Affinity Columns work?
They separate analytes through selective and reversible target–ligand interaction. The exact behavior depends on the selected phase and method conditions.
Which Affinity Columns should I choose?
Choose first by separation mechanism and named phase, then by particle size, pore size where listed, internal diameter, length, sample load and system pressure capability.
Does this page show the catalog configurations for Affinity Columns?
Yes. The searchable specification table presents the configurations included in the website catalog data for this family. Use the catalog number when requesting confirmation or a quotation.
What applications are suitable for Affinity Columns?
This family is relevant to targeted capture, cleanup, purification and analytical workflows. Final suitability depends on the analyte, sample matrix and method requirements.
Who supplies Affinity Columns in India?
Akira Analytical Solutions Pvt. Ltd. supplies SEPAX products in India and assists with catalog identification, column selection and technical enquiries.
Technical support for Affinity Columns
Send the application, current method and preferred dimensions to Akira Analytical Solutions Pvt. Ltd..