FPLC Empty Columns
SEPAX FPLC Empty Columns support user-packed FPLC workflows for laboratories packing their own compatible chromatography media. Use the searchable catalog table below to compare the listed phases, particle sizes, pore sizes, dimensions and catalog numbers, then confirm the final configuration against the intended method and instrument requirements.
FPLC Empty Columns specifications
| Product phase | Particle size (µm) | Dimensions | Catalog number |
|---|---|---|---|
| Generik FPLC Empty Column AF | 6.6 × 250 mm | 202000-0625-AF | |
| Generik FPLC Empty Column AF | 6.6 × 150 mm | 202000-0615-AF | |
| Generik FPLC Empty Column AF | 6.6 × 100 mm | 202000-0610-AF | |
| Generik FPLC Empty Column AF | 50 × 500 mm | 202000-5050-AF | |
| Generik FPLC Empty Column AF | 50 × 400 mm | 202000-5040-AF | |
| Generik FPLC Empty Column AF | 50 × 150 mm | 202000-5015-AF | |
| Generik FPLC Empty Column AF | 5 × 400 mm | 202000-0540-AF | |
| Generik FPLC Empty Column AF | 35 × 400 mm | 202000-3540-AF | |
| Generik FPLC Empty Column AF | 25 × 500 mm | 202000-2550-AF | |
| Generik FPLC Empty Column AF | 25 × 400 mm | 202000-2540-AF | |
| Generik FPLC Empty Column AF | 25 × 250 mm | 202000-2525-AF | |
| Generik FPLC Empty Column AF | 25 × 150 mm | 202000-2515-AF | |
| Generik FPLC Empty Column AF | 15 × 500 mm | 202000-1550-AF | |
| Generik FPLC Empty Column AF | 15 × 250 mm | 202000-1525-AF | |
| Generik FPLC Empty Column AF | 15 × 150 mm | 202000-1515-AF | |
| Generik FPLC Empty Column AF | 15 × 100 mm | 202000-1510-AF | |
| Generik FPLC Empty Column AF | 10 × 100 mm | 202000-1010-AF | |
| Generik FPLC Empty Column AF | 10 × 250 mm | 202000-1025-AF | |
| Generik FPLC Empty Column AF | 10 × 150 mm | 202000-1015-AF | |
| Generik FPLC Empty Column AA | 6.6 × 400 mm | 202000-0640-AA | |
| Generik FPLC Empty Column AA | 6.6 × 250 mm | 202000-0625-AA | |
| Generik FPLC Empty Column AA | 6.6 × 150 mm | 202000-0615-AA | |
| Generik FPLC Empty Column AA | 6.6 × 100 mm | 202000-0610-AA | |
| Generik FPLC Empty Column AA | 50 × 500 mm | 202000-5050-AA | |
| Generik FPLC Empty Column AA | 50 × 400 mm | 202000-5040-AA | |
| Generik FPLC Empty Column AA | 25 × 500 mm | 202000-2550-AA | |
| Generik FPLC Empty Column AA | 25 × 250 mm | 202000-2525-AA | |
| Generik FPLC Empty Column AA | 15 × 400 mm | 202000-1540-AA | |
| Generik FPLC Empty Column AA | 15 × 250 mm | 202000-1525-AA | |
| Generik FPLC Empty Column AA | 10 × 500 mm | 202000-1050-AA | |
| Generik FPLC Empty Column AA | 10 × 400 mm | 202000-1040-AA | |
| Generik FPLC Empty Column AA | 10 × 250 mm | 202000-1025-AA |
Overview
Named phases: Generik FPLC Empty Column AA, Generik FPLC Empty Column AF
Separation categories: FPLC Empty Column
Available particle sizes: See product table µm
Scientific principle
Selection should follow the separation mode and exact catalog configuration.
For detailed surface chemistry and packing guidance, contact technical support.
What are SEPAX FPLC Empty Columns?
SEPAX FPLC Empty Columns are catalog-listed products for user-packed FPLC workflows. The family is intended for laboratories packing their own compatible chromatography media, with available configurations shown in the specification table on this page.
How do FPLC Empty Columns work?
The separation is governed by the separation mechanism of the media selected and packed by the user. Selectivity also depends on the exact phase, mobile phase, sample properties, temperature, flow rate and column dimensions.
How to select a configuration
Start with the required separation mechanism and named phase. Then compare particle size, pore size where listed, internal diameter, length and catalog number. Match the configuration to system pressure capability, sample load and the scale of the method.
Applications
This family is relevant to protein purification development and custom preparative workflows. Suitability must be confirmed experimentally with the actual sample and method conditions.
Method development
Begin with a documented method or a chemically reasonable starting condition for the selected phase. Control sample solvent strength, buffer compatibility, pH, ionic strength, gradient profile, temperature and injection volume. Change one variable at a time and record system-suitability results.
Column care and storage
Filter samples and mobile phases, prevent buffer precipitation, avoid sudden solvent incompatibility and stay within the product-specific operating limits supplied with the column. After use, remove strongly retained material with a compatible wash and store the column only in a suitable solvent for that exact phase.
Replacement and method transfer
Do not select a replacement by dimensions alone. Compare separation mechanism, stationary-phase type, particle size, pore size where applicable, column geometry and method conditions. Verify equivalence through retention, resolution, efficiency, tailing and system-suitability testing.
For catalog identification and application support, contact SEPAX technical support in India.
Troubleshooting guidance
Channeling, voids, uneven bed height and excessive pressure usually point to packing quality, frit blockage, trapped air or inappropriate flow during bed formation.
Cleaning and regeneration
After unpacking or repacking, clean hardware thoroughly and inspect frits, seals and end fittings. Store dry or filled according to the material and resin requirements.
Mobile phase recommendations
Mobile-phase recommendations depend on the resin packed by the user. Select tubing, frits and hardware compatible with the intended buffer, pressure and biomolecule application.
Related SEPAX product families
For complementary selectivity or workflow steps, also review Proteomix Columns, Affinity Columns and Accessories. These related families can support orthogonal method development, sample cleanup, confirmation or scale-up depending on the application.
Frequently asked questions
What are FPLC Empty Columns?
FPLC Empty Columns are SEPAX catalog products used for user-packed FPLC workflows. The listed configurations can be compared by phase, particle size, pore size where applicable, dimensions and catalog number.
How do FPLC Empty Columns work?
They separate analytes through the separation mechanism of the media selected and packed by the user. The exact behavior depends on the selected phase and method conditions.
Which FPLC Empty Columns should I choose?
Choose first by separation mechanism and named phase, then by particle size, pore size where listed, internal diameter, length, sample load and system pressure capability.
Does this page show the catalog configurations for FPLC Empty Columns?
Yes. The searchable specification table presents the configurations included in the website catalog data for this family. Use the catalog number when requesting confirmation or a quotation.
What applications are suitable for FPLC Empty Columns?
This family is relevant to protein purification development and custom preparative workflows. Final suitability depends on the analyte, sample matrix and method requirements.
Who supplies FPLC Empty Columns in India?
Akira Analytical Solutions Pvt. Ltd. supplies SEPAX products in India and assists with catalog identification, column selection and technical enquiries.
Technical support for FPLC Empty Columns
Send the application, current method and preferred dimensions to Akira Analytical Solutions Pvt. Ltd..