Tween Columns
SEPAX Tween Columns support polysorbate-focused chromatography for samples requiring Tween or polysorbate analysis or selective handling. Use the searchable catalog table below to compare the listed phases, particle sizes, pore sizes, dimensions and catalog numbers, then confirm the final configuration against the intended method and instrument requirements.
Tween Columns specifications
| Product phase | Particle size (µm) | Dimensions | Catalog number |
|---|---|---|---|
| TweenTrap | 5 | 7.8 × 50 mm | 010054-7805 |
Overview
Named phases: TweenTrap
Separation categories: Tween
Available particle sizes: 5 µm
Scientific principle
Selection should follow the separation mode and exact catalog configuration.
For detailed surface chemistry and packing guidance, contact technical support.
What are SEPAX Tween Columns?
SEPAX Tween Columns are catalog-listed products for polysorbate-focused chromatography. The family is intended for samples requiring Tween or polysorbate analysis or selective handling, with available configurations shown in the specification table on this page.
How do Tween Columns work?
The separation is governed by the mechanism represented by the selected Tween or TweenTrap product. Selectivity also depends on the exact phase, mobile phase, sample properties, temperature, flow rate and column dimensions.
How to select a configuration
Start with the required separation mechanism and named phase. Then compare particle size, pore size where listed, internal diameter, length and catalog number. Match the configuration to system pressure capability, sample load and the scale of the method.
Applications
This family is relevant to polysorbate-related analytical and sample-preparation workflows. Suitability must be confirmed experimentally with the actual sample and method conditions.
Method development
Begin with a documented method or a chemically reasonable starting condition for the selected phase. Control sample solvent strength, buffer compatibility, pH, ionic strength, gradient profile, temperature and injection volume. Change one variable at a time and record system-suitability results.
Column care and storage
Filter samples and mobile phases, prevent buffer precipitation, avoid sudden solvent incompatibility and stay within the product-specific operating limits supplied with the column. After use, remove strongly retained material with a compatible wash and store the column only in a suitable solvent for that exact phase.
Replacement and method transfer
Do not select a replacement by dimensions alone. Compare separation mechanism, stationary-phase type, particle size, pore size where applicable, column geometry and method conditions. Verify equivalence through retention, resolution, efficiency, tailing and system-suitability testing.
For catalog identification and application support, contact SEPAX technical support in India.
Troubleshooting guidance
Carryover, baseline disturbance and changing response can result from surfactant adsorption, incomplete washing, detector contamination or inconsistent sample dilution.
Cleaning and regeneration
Use compatible washes to remove retained surfactant material and prevent carryover. Flush the system promptly after concentrated samples and avoid microbial contamination in aqueous mobile phases.
Mobile phase recommendations
Choose the mobile phase according to the target polysorbate components and detection approach. Maintain consistent solvent composition, temperature and sample preparation because these complex mixtures can be sensitive to method variation.
Related SEPAX product families
For complementary selectivity or workflow steps, also review HP Columns, HILIC Columns and GP Columns. These related families can support orthogonal method development, sample cleanup, confirmation or scale-up depending on the application.
Frequently asked questions
What are Tween Columns?
Tween Columns are SEPAX catalog products used for polysorbate-focused chromatography. The listed configurations can be compared by phase, particle size, pore size where applicable, dimensions and catalog number.
How do Tween Columns work?
They separate analytes through the mechanism represented by the selected Tween or TweenTrap product. The exact behavior depends on the selected phase and method conditions.
Which Tween Columns should I choose?
Choose first by separation mechanism and named phase, then by particle size, pore size where listed, internal diameter, length, sample load and system pressure capability.
Does this page show the catalog configurations for Tween Columns?
Yes. The searchable specification table presents the configurations included in the website catalog data for this family. Use the catalog number when requesting confirmation or a quotation.
What applications are suitable for Tween Columns?
This family is relevant to polysorbate-related analytical and sample-preparation workflows. Final suitability depends on the analyte, sample matrix and method requirements.
Who supplies Tween Columns in India?
Akira Analytical Solutions Pvt. Ltd. supplies SEPAX products in India and assists with catalog identification, column selection and technical enquiries.
Technical support for Tween Columns
Send the application, current method and preferred dimensions to Akira Analytical Solutions Pvt. Ltd..