Product family

Ion Exchange Columns

SEPAX ion exchange columns separate charged proteins, antibodies, peptides, nucleic acids and related biomolecules through reversible electrostatic interactions. Use the searchable catalog table below to compare catalog-listed strong and weak anion- and cation-exchange phases, particle sizes, pore sizes, dimensions and catalog numbers.

Catalog configurations

Ion Exchange Columns specifications

Product phase	Particle size (µm)	Pore size (Å)	Dimensions	Catalog number
Proteomix POR-Q	15	1000	2.1 × 35 mm	405950-2103
Proteomix WCX	1.7		2 × 10 mm	402NP2-2001
Proteomix POR-Q	15	1000	4.6 × 50 mm	405950-4605
Proteomix POR S	30	1000	4.6 × 50 mm	221130950-4605
Proteomix POR Q	30	1000	4.6 × 50 mm	221430950-4605
Proteomix POR Q	15	1000	4.6 × 50 mm	221115950-4605
Proteomix POR15-S	15	1000	10 × 250 mm	221115950-10025
Proteomix POR15-S	15	1000	10 × 150 mm	221115950-10015
Proteomix POR15-Q	15	1000	10 × 250 mm	221415950-10025
Proteomix POR15-Q	15	1000	10 × 150 mm	221415950-10015
Proteomix POR15-HQ	15	1000	7.8 × 250 mm	222215950-7825
Proteomix POR15-HQ	15	1000	7.8 × 150 mm	222215950-7815
Proteomix POR15-HQ	15	1000	7.8 × 100 mm	222215950-7810
Proteomix POR15-HQ	15	1000	50 × 250 mm	222215950-50025
Proteomix POR15-HQ	15		50 × 150 mm	222215950-50015
Proteomix POR15-HQ	15	1000	50 × 100 mm	222215950-50010
Proteomix POR15-HQ	15	1000	4.6 × 250 mm	222215950-4625
Proteomix POR15-HQ	15	1000	4.6 × 150 mm	222215950-4615
Proteomix POR15-HQ	15	1000	4.6 × 100 mm	222215950-4610
Proteomix POR15-HQ	15	1000	30 × 250 mm	222215950-30025
Proteomix POR15-HQ	15	1000	30 × 150 mm	222215950-30015
Proteomix POR15-HQ	15	1000	30 × 100 mm	222215950-30010
Proteomix POR15-HQ	15	1000	21.2 × 250 mm	222215950-21225
Proteomix POR15-HQ	15	1000	21.2 × 150 mm	222215950-21215
Proteomix POR15-HQ	15	1000	21.2 × 100 mm	222215950-21210
Proteomix POR15-HQ	15		10 × 250 mm	222215950-10025
Proteomix POR15-HQ	15	1000	10 × 150 mm	222215950-10015
Proteomix POR15-HQ	15	1000	10 × 100 mm	222215950-10010
Proteomix SAX	5		4 × 10 mm	403NP5-4001
Proteomix WCX	5		2 × 10 mm	402NP5-2001
Proteomix WCX	5		7.8 × 50 mm	402NP5-7805
Proteomix WCX	5		7.8 × 250 mm	402NP5-7825
Proteomix WCX	5		7.8 × 150 mm	402NP5-7815
Proteomix WCX	5		7.8 × 100 mm	402NP5-7810
Proteomix WCX	5		4.6 × 50 mm	402NP5-4605
Proteomix WCX	5		4.6 × 30 mm	402NP5-4603
Proteomix WCX	5		4.6 × 250 mm	402NP5-4625
Proteomix WCX	5		4.6 × 150 mm	402NP5-4615
Proteomix WCX	5		4.6 × 100 mm	402NP5-4610
Proteomix WCX	5		4 × 10 mm	402NP5-4001
Proteomix WCX	5		30 × 50 mm	402NP5-30005
Proteomix WCX	5		30 × 250 mm	402NP5-30025
Proteomix WCX	5		30 × 150 mm	402NP5-30015
Proteomix WCX	5		30 × 100 mm	402NP5-30010
Proteomix WCX	5		21.2 × 50 mm	402NP5-21205
Proteomix WCX	5		21.2 × 250 mm	402NP5-21225
Proteomix WCX	5		21.2 × 150 mm	402NP5-21215
Proteomix WCX	5		2.1 × 50 mm	402NP5-2105
Proteomix WCX	5		2.1 × 30 mm	402NP5-2103
Proteomix WCX	5		2.1 × 250 mm	402NP5-2125
Proteomix WCX	5		2.1 × 150 mm	402NP5-2115
Proteomix WCX	5		2.1 × 100 mm	402NP5-2110
Proteomix WCX	5		10 × 250 mm	402NP5-10025
Proteomix WCX	5		4.6 × 50 mm	402NP5P-4605
Proteomix WCX	5		4.6 × 250 mm	402NP5P-4625
Proteomix WCX	5		4.6 × 150 mm	402NP5P-4615
Proteomix WCX	5		2.1 × 50 mm	402NP5P-2105
Proteomix WCX	5		2.1 × 250 mm	402NP5P-2125
Proteomix WCX	5		2.1 × 150 mm	402NP5P-2115
Proteomix WCX	3		4.6 × 50 mm	402NP3-4605
Proteomix WCX	3		4.6 × 30 mm	402NP3-4603
Proteomix WCX	3		4.6 × 150 mm	402NP3-4615
Proteomix WCX	3		4.6 × 100 mm	402NP3-4610
Proteomix WCX	3		4 × 10 mm	402NP3-4001
Proteomix WCX	3		2.1 × 50 mm	402NP3-2105
Proteomix WCX	3		2.1 × 30 mm	402NP3-2103
Proteomix WCX	3		2.1 × 100 mm	402NP3-2110
Proteomix WCX	3		2 × 10 mm	402NP3-2001
Proteomix WCX	10		7.8 × 50 mm	402NP10-7805
Proteomix WCX	10		7.8 × 250 mm	402NP10-7825
Proteomix WCX	10		7.8 × 150 mm	402NP10-7815
Proteomix WCX	10		7.8 × 100 mm	402NP10-7810
Proteomix WCX	10		4.6 × 50 mm	402NP10-4605
Proteomix WCX	10		4.6 × 30 mm	402NP10-4603
Proteomix WCX	10		4.6 × 250 mm	402NP10-4625
Proteomix WCX	10		4.6 × 150 mm	402NP10-4615
Proteomix WCX	10		4.6 × 100 mm	402NP10-4610
Proteomix WCX	10		30 × 50 mm	402NP10-30005
Proteomix WCX	10		30 × 250 mm	402NP10-30025
Proteomix WCX	10		30 × 150 mm	402NP10-30015
Proteomix WCX	10		30 × 100 mm	402NP10-30010
Proteomix WCX	10		21.2 × 50 mm	402NP10-21205
Proteomix WCX	10		21.2 × 250 mm	402NP10-21225
Proteomix WCX	10		21.2 × 150 mm	402NP10-21215
Proteomix WCX	10		2.1 × 50 mm	402NP10-2105
Proteomix WCX	10		2.1 × 30 mm	402NP10-2103
Proteomix WCX	10		2.1 × 250 mm	402NP10-2125
Proteomix WCX	10		2.1 × 150 mm	402NP10-2115
Proteomix WCX	10		2.1 × 100 mm	402NP10-2110
Proteomix WCX	10		10 × 50 mm	402NP10-10005
Proteomix WCX	10		10 × 250 mm	402NP10-10025
Proteomix WCX	10		10 × 100 mm	402NP10-10010
Proteomix WCX	10		4.6 × 50 mm	402NP10P-4605
Proteomix WCX	10		4.6 × 250 mm	402NP10P-4625
Proteomix WCX	10		2.1 × 50 mm	402Np10P-2105
Proteomix WCX	10		2.1 × 250 mm	402NP10P-2125
Proteomix WCX	1.7		4.6 × 50 mm	402NP2-4605
Proteomix WCX	1.7		4.6 × 30 mm	402NP2-4603
Proteomix WCX	1.7		4.6 × 150 mm	402NP2-4615
Proteomix WCX	1.7		4.6 × 100 mm	402NP2-4610
Proteomix WCX	1.7		4 × 10 mm	402NP2-4001
Proteomix WCX	1.7		2.1 × 50 mm	402NP2-2105
Proteomix WCX	1.7		2.1 × 30 mm	402NP2-2103
Proteomix WCX	1.7		2.1 × 150 mm	402NP2-2115
Proteomix WCX	1.7		2.1 × 100 mm	402NP2-2110
Proteomix WCX	1.7		4.6 × 50 mm	402NP2P-4605
Proteomix WCX	1.7		2.1 × 50 mm	402NP2P-2105
Proteomix WCX	5		4 × 10 mm	402NP5-4001C
Proteomix WCX	5		2 × 10 mm	402NP5-2001C
Proteomix WCX	5		10 × 50 mm	402NP5-10005
Proteomix WCX	3		4 × 10 mm	402NP3-4001C
Proteomix WCX	3		2 × 10 mm	402NP3-2001C
Proteomix WCX	10		4 × 10 mm	402NP10-4001C
Proteomix WCX	10		2 × 10 mm	402NP10-2001C
Proteomix WCX	10		4 × 10 mm	402NP10-4001
Proteomix WCX	10		2 × 10 mm	402NP10-2001
Proteomix WCX	1.7		4 × 10 mm	402NP2-4001C
Proteomix WCX	1.7		2 × 10 mm	402NP2-2001C
Proteomix WCX	3		2.1 × 150 mm	402NP3-2115
Proteomix WCX	3		4.6 × 50 mm	402NP3P-4605
Proteomix WCX	3		2.1 × 50 mm	402NP3P-2105
Proteomix WAX	5		7.8 × 50 mm	404NP5-7805
Proteomix WAX	5		7.8 × 250 mm	404NP5-7825
Proteomix WAX	5		7.8 × 150 mm	404NP5-7815
Proteomix WAX	5		7.8 × 100 mm	404NP5-7810
Proteomix WAX	5		4.6 × 50 mm	404NP5-4605
Proteomix WAX	5		4.6 × 30 mm	404NP5-4603
Proteomix WAX	5		4.6 × 250 mm	404NP5-4625
Proteomix WAX	5		4.6 × 150 mm	404NP5-4615
Proteomix WAX	5		4.6 × 100 mm	404NP5-4610
Proteomix WAX	5		30 × 50 mm	404NP5-30005
Proteomix WAX	5		30 × 250 mm	404NP5-30025
Proteomix WAX	5		30 × 150 mm	404NP5-30015
Proteomix WAX	5		30 × 100 mm	404NP5-30010
Proteomix WAX	5		21.2 × 50 mm	404NP5-21205
Proteomix WAX	5		21.2 × 250 mm	404NP5-21225
Proteomix WAX	5		21.2 × 150 mm	404NP5-21215
Proteomix WAX	5		2.1 × 50 mm	404NP5-2105
Proteomix WAX	5		2.1 × 30 mm	404NP5-2103
Proteomix WAX	5		2.1 × 250 mm	404NP5-2125
Proteomix WAX	5		2.1 × 150 mm	404NP5-2115
Proteomix WAX	5		2.1 × 100 mm	404NP5-2110
Proteomix WAX	5		10 × 250 mm	404NP5-10025
Proteomix WAX	5		4.6 × 50 mm	404NP5P-4605
Proteomix WAX	5		4.6 × 250 mm	404NP5P-4625
Proteomix WAX	5		4.6 × 150 mm	404NP5P-4615
Proteomix WAX	5		2.1 × 50 mm	404NP5P-2105
Proteomix WAX	5		2.1 × 250 mm	404NP5P-2125
Proteomix WAX	5		2.1 × 150 mm	404NP5P-2115
Proteomix WAX	3		7.8 × 100 mm	404NP3-7810
Proteomix WAX	3		4.6 × 50 mm	404NP3-4605
Proteomix WAX	3		4.6 × 30 mm	404NP3-4603
Proteomix WAX	3		4.6 × 150 mm	404NP3-4615
Proteomix WAX	3		4.6 × 100 mm	404NP3-4610
Proteomix WAX	3		2.1 × 50 mm	404NP3-2105
Proteomix WAX	3		2.1 × 30 mm	404NP3-2103
Proteomix WAX	3		2.1 × 100 mm	404NP3-2110
Proteomix WAX	10		7.8 × 50 mm	404NP10-7805
Proteomix WAX	10		7.8 × 250 mm	404NP10-7825
Proteomix WAX	10		7.8 × 150 mm	404NP10-7815
Proteomix WAX	10		7.8 × 100 mm	404NP10-7810
Proteomix WAX	10		4.6 × 50 mm	404NP10-4605
Proteomix WAX	10		4.6 × 30 mm	404NP10-4603
Proteomix WAX	10		4.6 × 250 mm	404NP10-4625
Proteomix WAX	10		4.6 × 150 mm	404NP10-4615
Proteomix WAX	10		4.6 × 100 mm	404NP10-4610
Proteomix WAX	10		30 × 50 mm	404NP10-30005
Proteomix WAX	10		30 × 250 mm	404NP10-30025
Proteomix WAX	10		30 × 150 mm	404NP10-30015
Proteomix WAX	10		30 × 100 mm	404NP10-30010
Proteomix WAX	10		21.2 × 50 mm	404NP10-21205
Proteomix WAX	10		21.2 × 250 mm	404NP10-21225
Proteomix WAX	10		21.2 × 150 mm	404NP10-21215
Proteomix WAX	10		2.1 × 50 mm	404NP10-2105
Proteomix WAX	10		2.1 × 30 mm	404NP10-2103
Proteomix WAX	10		2.1 × 250 mm	404NP10-2125
Proteomix WAX	10		2.1 × 150 mm	404NP10-2115
Proteomix WAX	10		2.1 × 100 mm	404NP10-2110
Proteomix WAX	10		10 × 50 mm	404NP10-10005
Proteomix WAX	10		10 × 250 mm	404NP10-10025
Proteomix WAX	10		10 × 100 mm	404NP10-10010
Proteomix WAX	10		4.6 × 50 mm	404NP10P-4605
Proteomix WAX	10		4.6 × 250 mm	404NP10P-4625
Proteomix WAX	10		2.1 × 50 mm	404NP10P-2105
Proteomix WAX	10		2.1 × 250 mm	404NP10P-2125
Proteomix WAX	1.7		4.6 × 50 mm	404NP2-4605
Proteomix WAX	1.7		4.6 × 30 mm	404NP2-4603
Proteomix WAX	1.7		4.6 × 150 mm	404NP2-4615
Proteomix WAX	1.7		4.6 × 100 mm	404NP2-4610
Proteomix WAX	1.7		4 × 10 mm	404NP2-4001
Proteomix WAX	1.7		2.1 × 50 mm	404NP2-2105
Proteomix WAX	1.7		2.1 × 30 mm	404NP2-2103
Proteomix WAX	1.7		2.1 × 150 mm	404NP2-2115
Proteomix WAX	1.7		2.1 × 100 mm	404NP2-2110
Proteomix WAX	1.7		2 × 10 mm	404NP2-2001
Proteomix WAX	1.7		4.6 × 50 mm	404NP2P-4605
Proteomix WAX	1.7		2.1 × 50 mm	404NP2P-2105
Proteomix WAX	5		4 × 10 mm	404NP5-4001
Proteomix WAX	5		4 × 10 mm	404NP5-4001C
Proteomix WAX	5		2 × 10 mm	404NP5-2001C
Proteomix WAX	5		2 × 10 mm	404NP5-2001
Proteomix WAX	5		10 × 50 mm	404NP5-10005
Proteomix WAX	3		4 × 10 mm	404NP3-4001
Proteomix WAX	3		4 × 10 mm	404NP3-4001C
Proteomix WAX	3		2 × 10 mm	404NP3-2001C
Proteomix WAX	3		2 × 10 mm	404NP3-2001
Proteomix WAX	10		4 × 10 mm	404NP10-4001C
Proteomix WAX	10		2 × 10 mm	404NP10-2001C
Proteomix WAX	10		4 × 10 mm	404NP10-4001
Proteomix WAX	10		2 × 10 mm	404NP10-2001
Proteomix WAX	1.7		4 × 10 mm	404NP2-4001C
Proteomix WAX	1.7		2 × 10 mm	404NP2-2001C
Proteomix WAX	3		2.1 × 150 mm	404NP3-2115
Proteomix WAX	3		4.6 × 50 mm	404NP3P-4605
Proteomix WAX	3		2.1 × 50 mm	404NP3P-2105
Proteomix SCX	5		4.6 × 250 mm	401NP5-4625
Proteomix SCX	5		4.6 × 150 mm	401NP5-4615
Proteomix SCX	5		4.6 × 100 mm	401NP5-4610
Proteomix SCX	5		2.1 × 150 mm	401NP5-2115
Proteomix SCX	5		7.8 × 50 mm	401NP5-7805
Proteomix SCX	5		7.8 × 250 mm	401NP5-7825
Proteomix SCX	5		7.8 × 150 mm	401NP5-7815
Proteomix SCX	5		7.8 × 100 mm	401NP5-7810
Proteomix SCX	5		4.6 × 50 mm	401NP5-4605
Proteomix SCX	5		4.6 × 30 mm	401NP5-4603
Proteomix SCX	5		30 × 50 mm	401NP5-30005
Proteomix SCX	5		30 × 250 mm	401NP5-30025
Proteomix SCX	5		30 × 150 mm	401NP5-30015
Proteomix SCX	5		30 × 100 mm	401NP5-30010
Proteomix SCX	5		21.2 × 50 mm	401NP5-21205
Proteomix SCX	5		21.2 × 250 mm	401NP5-21225
Proteomix SCX	5		21.2 × 150 mm	401NP5-21215
Proteomix SCX	5		2.1 × 50 mm	401NP5-2105
Proteomix SCX	5		2.1 × 30 mm	401NP5-2103
Proteomix SCX	5		2.1 × 250 mm	401NP5-2125
Proteomix SCX	5		2.1 × 100 mm	401NP5-2110
Proteomix SCX	5		10 × 250 mm	401NP5-10025
Proteomix SCX	5		4.6 × 50 mm	401NP5P-4605
Proteomix SCX	5		4.6 × 250 mm	401NP5P-4625
Proteomix SCX	5		4.6 × 150 mm	401NP5P-4615
Proteomix SCX	5		2.1 × 250 mm	401NP5P-2125
Proteomix SCX	5		2.1 × 150 mm	401NP5P-2115
Proteomix SCX	5		2.1 × 50 mm	401NP5P-2105
Proteomix SCX	3		4.6 × 150 mm	401NP3-4615
Proteomix SCX	3		4.6 × 100 mm	401NP3-4610
Proteomix SCX	3		4.6 × 50 mm	401NP3-4605
Proteomix SCX	3		4.6 × 30 mm	401NP3-4603
Proteomix SCX	3		2.1 × 50 mm	401NP3-2105
Proteomix SCX	3		2.1 × 30 mm	401NP3-2103
Proteomix SCX	3		2.1 × 100 mm	401NP3-2110
Proteomix SCX	10		4.6 × 50 mm	401NP10-4605
Proteomix SCX	10		4.6 × 250 mm	401NP10-4625
Proteomix SCX	10		7.8 × 50 mm	401NP10-7805
Proteomix SCX	10		7.8 × 300 mm	401NP10-7830
Proteomix SCX	10		7.8 × 250 mm	401NP10-7825
Proteomix SCX	10		7.8 × 150 mm	401NP10-7815
Proteomix SCX	10		7.8 × 100 mm	401NP10-7810
Proteomix SCX	10		4.6 × 30 mm	401NP10-4603
Proteomix SCX	10		4.6 × 150 mm	401NP10-4615
Proteomix SCX	10		4.6 × 100 mm	401NP10-4610
Proteomix SCX	10		30 × 50 mm	401NP10-30005
Proteomix SCX	10		30 × 250 mm	401NP10-30025
Proteomix SCX	10		30 × 150 mm	401NP10-30015
Proteomix SCX	10		30 × 100 mm	401NP10-30010
Proteomix SCX	10		21.2 × 50 mm	401NP10-21205
Proteomix SCX	10		21.2 × 250 mm	401NP10-21225
Proteomix SCX	10		21.2 × 150 mm	401NP10-21215
Proteomix SCX	10		2.1 × 50 mm	401NP10-2105
Proteomix SCX	10		2.1 × 30 mm	401NP10-2103
Proteomix SCX	10		2.1 × 250 mm	401NP10-2125
Proteomix SCX	10		2.1 × 150 mm	401NP10-2115
Proteomix SCX	10		2.1 × 100 mm	401NP10-2110
Proteomix SCX	10		10 × 50 mm	401NP10-10005
Proteomix SCX	10		10 × 250 mm	401NP10-10025
Proteomix SCX	10		10 × 100 mm	401NP10-10010
Proteomix SCX	10		4.6 × 50 mm	401NP10P-4605
Proteomix SCX	10		4.6 × 250 mm	401NP10P-4625
Proteomix SCX	10		2.1 × 50 mm	401NP10P-2105
Proteomix SCX	10		2.1 × 250 mm	401NP10P-2125
Proteomix SCX	1.7		4.6 × 150 mm	401NP2-4615
Proteomix SCX	1.7		4.6 × 100 mm	401NP2-4610
Proteomix SCX	1.7		2.1 × 50 mm	401NP2-2105
Proteomix SCX	1.7		2.1 × 150 mm	401NP2-2115
Proteomix SCX	1.7		4.6 × 50 mm	401NP2-4605
Proteomix SCX	1.7		4.6 × 30 mm	401NP2-4603
Proteomix SCX	1.7		4 × 10 mm	401NP2-4001
Proteomix SCX	1.7		2.1 × 30 mm	401NP2-2103
Proteomix SCX	1.7		2.1 × 100 mm	401NP2-2110
Proteomix SCX	1.7		2 × 10 mm	401NP2-2001
Proteomix SCX	1.7		4.6 × 50 mm	401NP2P-4605
Proteomix SCX	1.7		2.1 × 50 mm	401NP2P-2105
Proteomix SCX	5		4 × 10 mm	401NP5-4001C
Proteomix SCX	5		2 × 10 mm	401NP5-2001C
Proteomix SCX	5		2 × 10 mm	401NP5-2001
Proteomix SCX	5		4 × 10 mm	401NP5-4001
Proteomix SCX	5		10 × 50 mm	401NP5-10005
Proteomix SCX	3		4 × 10 mm	401NP3-4001C
Proteomix SCX	3		2 × 10 mm	401NP3-2001C
Proteomix SCX	3		2 × 10 mm	401NP3-2001
Proteomix SCX	3		4 × 10 mm	401NP3-4001
Proteomix SCX	10		4 × 10 mm	401NP10-4001C
Proteomix SCX	10		2 × 10 mm	401NP10-2001C
Proteomix SCX	10		4 × 10 mm	401NP10-4001
Proteomix SCX	10		2 × 10 mm	401NP10-2001
Proteomix SCX	1.7		4 × 10 mm	401NP2-4001C
Proteomix SCX	1.7		2 × 10 mm	401NP2-2001C
Proteomix SCX	3		2.1 × 150 mm	401NP3-2115
Proteomix SCX	3		4.6 × 50 mm	401NP3P-4605
Proteomix SCX	3		2.1 × 50 mm	401NP3P-2105
Proteomix SAX	5		7.8 × 50 mm	403NP5-7805
Proteomix SAX	5		7.8 × 250 mm	403NP5-7825
Proteomix SAX	5		7.8 × 150 mm	403NP5-7815
Proteomix SAX	5		7.8 × 100 mm	403NP5-7810
Proteomix SAX	5		4.6 × 50 mm	403NP5-4605
Proteomix SAX	5		4.6 × 30 mm	403NP5-4603
Proteomix SAX	5		4.6 × 250 mm	403NP5-4625
Proteomix SAX	5		4.6 × 150 mm	403NP5-4615
Proteomix SAX	5		4.6 × 100 mm	403NP5-4610
Proteomix SAX	5		30 × 50 mm	403NP5-30005
Proteomix SAX	5		30 × 250 mm	403NP5-30025
Proteomix SAX	5		30 × 150 mm	403NP5-30015
Proteomix SAX	5		30 × 100 mm	403NP5-30010
Proteomix SAX	5		21.2 × 50 mm	403NP5-21205
Proteomix SAX	5		21.2 × 250 mm	403NP5-21225
Proteomix SAX	5		21.2 × 150 mm	403NP5-21215
Proteomix SAX	5		2.1 × 50 mm	403NP5-2105
Proteomix SAX	5		2.1 × 30 mm	403NP5-2103
Proteomix SAX	5		2.1 × 250 mm	403NP5-2125
Proteomix SAX	5		2.1 × 150 mm	403NP5-2115
Proteomix SAX	5		2.1 × 100 mm	403NP5-2110
Proteomix SAX	5		10 × 250 mm	403NP5-10025
Proteomix SAX	5		4.6 × 50 mm	403NP5P-4605
Proteomix SAX	5		4.6 × 250 mm	403NP5P-4625
Proteomix SAX	5		4.6 × 150 mm	403NP5P-4615
Proteomix SAX	5		2.1 × 50 mm	403NP5P-2105
Proteomix SAX	5		2.1 × 250 mm	403NP5P-2125
Proteomix SAX	5		2.1 × 150 mm	403NP5P-2115
Proteomix SAX	3		7.8 × 150 mm	403NP3-7815
Proteomix SAX	3		4.6 × 50 mm	403NP3-4605
Proteomix SAX	3		4.6 × 30 mm	403NP3-4603
Proteomix SAX	3		4.6 × 150 mm	403NP3-4615
Proteomix SAX	3		4.6 × 100 mm	403NP3-4610
Proteomix SAX	3		2.1 × 50 mm	403NP3-2105
Proteomix SAX	3		2.1 × 30 mm	403NP3-2103
Proteomix SAX	3		2.1 × 100 mm	403NP3-2110
Proteomix SAX	10		7.8 × 50 mm	403NP10-7805
Proteomix SAX	10		7.8 × 250 mm	403NP10-7825
Proteomix SAX	10		7.8 × 150 mm	403NP10-7815
Proteomix SAX	10		7.8 × 100 mm	403NP10-7810
Proteomix SAX	10		50 × 250 mm	403NP10LL-50025
Proteomix SAX	10		50 × 250 mm	403NP10-50025
Proteomix SAX	10		4.6 × 50 mm	403NP10-4605
Proteomix SAX	10		4.6 × 30 mm	403NP10-4603
Proteomix SAX	10		4.6 × 250 mm	403NP10-4625
Proteomix SAX	10		4.6 × 150 mm	403NP10-4615
Proteomix SAX	10		4.6 × 100 mm	403NP10-4610
Proteomix SAX	10		30 × 50 mm	403NP10-30005
Proteomix SAX	10		30 × 250 mm	403NP10-30025
Proteomix SAX	10		30 × 150 mm	403NP10-30015
Proteomix SAX	10		30 × 100 mm	403NP10-30010
Proteomix SAX	10		21.2 × 50 mm	403NP10-21205
Proteomix SAX	10		21.2 × 250 mm	403NP10-21225
Proteomix SAX	10		21.2 × 150 mm	403NP10-21215
Proteomix SAX	10		2.1 × 50 mm	403NP10-2105
Proteomix SAX	10		2.1 × 30 mm	403NP10-2103
Proteomix SAX	10		2.1 × 250 mm	403NP10-2125
Proteomix SAX	10		2.1 × 150 mm	403NP10-2115
Proteomix SAX	10		2.1 × 100 mm	403NP10-2110
Proteomix SAX	10		10 × 50 mm	403NP10-10005
Proteomix SAX	10		10 × 250 mm	403NP10-10025
Proteomix SAX	10		10 × 100 mm	403NP10-10010
Proteomix SAX	10		4.6 × 50 mm	403NP10P-4605
Proteomix SAX	10		4.6 × 250 mm	403NP10P-4625
Proteomix SAX	10		2.1 × 50 mm	403NP10P-2105
Proteomix SAX	10		2.1 × 250 mm	403NP10P-2125
Proteomix SAX	1.7		4.6 × 50 mm	403NP2-4605
Proteomix SAX	1.7		4.6 × 30 mm	403NP2-4603
Proteomix SAX	1.7		4.6 × 150 mm	403NP2-4615
Proteomix SAX	1.7		4.6 × 100 mm	403NP2-4610
Proteomix SAX	1.7		4 × 10 mm	403NP2-4001
Proteomix SAX	1.7		2.1 × 50 mm	403NP2-2105
Proteomix SAX	1.7		2.1 × 30 mm	403NP2-2103
Proteomix SAX	1.7		2.1 × 150 mm	403NP2-2115
Proteomix SAX	1.7		2.1 × 100 mm	403NP2-2110
Proteomix SAX	1.7		2 × 10 mm	403NP2-2001
Proteomix SAX	1.7		4.6 × 50 mm	403NP2P-4605
Proteomix SAX	1.7		2.1 × 50 mm	403NP2P-2105
Proteomix SAX	5		4 × 10 mm	403NP5-4001C
Proteomix SAX	5		2 × 10 mm	403NP5-2001C
Proteomix SAX	5		2 × 10 mm	403NP5-2001
Proteomix SAX	5		10 × 50 mm	403NP5-10005
Proteomix SAX	3		4 × 10 mm	403NP3-4001C
Proteomix SAX	3		2 × 10 mm	403NP3-2001C
Proteomix SAX	3		4 × 10 mm	403NP3-4001
Proteomix SAX	3		2 × 10 mm	403NP3-2001
Proteomix SAX	10		4 × 10 mm	403NP10-4001C
Proteomix SAX	10		2 × 10 mm	403NP10-2001C
Proteomix SAX	10		2 × 10 mm	403NP10-2001
Proteomix SAX	5		4 × 10 mm	403NP10-4001
Proteomix SAX	1.7		4 × 10 mm	403NP2-4001C
Proteomix SAX	1.7		2 × 10 mm	403NP2-2001C
Proteomix SAX	3		2.1 × 150 mm	403NP3-2115
Proteomix SAX	3		4.6 × 50 mm	403NP3P-4605
Proteomix SAX	3		4.6 × 150 mm	403NP3P-4615
Proteomix SAX	3		2.1 × 50 mm	403NP3P-2105
Proteomix SAX	3		4 × 10 mm	403NP3P-4001
Proteomix AAV-SAX	3		4.6 × 50 mm	411303P-4605
Proteomix AAV-SAX	3		4.6 × 100 mm	411303P-4610
Antibodix WCX	5		4.6 × 150 mm	602NP5P-4615
Antibodix WCX	10		10 × 50 mm	602NP10-10005
Antibodix WCX	5		4 × 10 mm	602NP5-4001C
Antibodix WCX	5		2 × 10 mm	602NP5-2001C
Antibodix WCX	5		10 × 50 mm	602NP5-10005
Antibodix WCX	3		4 × 10 mm	602NP3-4001C
Antibodix WCX	3		2 × 10 mm	602NP3-2001C
Antibodix WCX	10		4 × 10 mm	602NP10-4001C
Antibodix WCX	10		2 × 10 mm	602NP10-2001C
Antibodix WCX	1.7		4 × 10 mm	602NP2-4001C
Antibodix WCX	1.7		2 × 10 mm	602NP2-2001C
Antibodix WCX	5		4.6 × 250 mm	602NP5P-4625
Antibodix WCX	10		4.6 × 250 mm	602NP10-4625
Antibodix WCX	10		4.6 × 250 mm	602NP10P-4625
Antibodix WCX	5		2 × 10 mm	602NP5-2001
Antibodix WCX	5		7.8 × 50 mm	602NP5-7805
Antibodix WCX	5		7.8 × 250 mm	602NP5-7825
Antibodix WCX	5		7.8 × 150 mm	602NP5-7815
Antibodix WCX	5		7.8 × 100 mm	602NP5-7810
Antibodix WCX	5		4.6 × 50 mm	602NP5-4605
Antibodix WCX	5		4.6 × 30 mm	602NP5-4603
Antibodix WCX	5		4.6 × 250 mm	602NP5-4625
Antibodix WCX	5		4.6 × 150 mm	602NP5-4615
Antibodix WCX	5		4.6 × 100 mm	602NP5-4610
Antibodix WCX	5		4 × 10 mm	602NP5-4001
Antibodix WCX	5		30 × 50 mm	602NP5-30005
Antibodix WCX	5		30 × 250 mm	602NP5-30025
Antibodix WCX	5		30 × 150 mm	602NP5-30015
Antibodix WCX	5		30 × 100 mm	602NP5-30010
Antibodix WCX	5		21.2 × 50 mm	602NP5-21205
Antibodix WCX	5		21.2 × 250 mm	602NP5-21225
Antibodix WCX	5		21.2 × 150 mm	602NP5-21215
Antibodix WCX	5		2.1 × 50 mm	602NP5-2105
Antibodix WCX	5		2.1 × 30 mm	602NP5-2103
Antibodix WCX	5		2.1 × 250 mm	602NP5-2125
Antibodix WCX	5		2.1 × 150 mm	602NP5-2115
Antibodix WCX	5		2.1 × 100 mm	602NP5-2110
Antibodix WCX	5		10 × 250 mm	602NP5-10025
Antibodix WCX	5		4.6 × 50 mm	602NP5P-4605
Antibodix WCX	5		4.6 × 100 mm	602NP5P-4610
Antibodix WCX	5		2.1 × 50 mm	602NP5P-2105
Antibodix WCX	5		2.1 × 250 mm	602NP5P-2125
Antibodix WCX	3		4.6 × 150 mm	602NP3-4615
Antibodix WCX	3		4.6 × 50 mm	602NP3-4605
Antibodix WCX	3		4.6 × 30 mm	602NP3-4603
Antibodix WCX	3		4.6 × 100 mm	602NP3-4610
Antibodix WCX	3		4 × 10 mm	602NP3-4001
Antibodix WCX	3		2.1 × 50 mm	602NP3-2105
Antibodix WCX	3		2.1 × 30 mm	602NP3-2103
Antibodix WCX	3		2.1 × 100 mm	602NP3-2110
Antibodix WCX	3		2 × 10 mm	602NP3-2001
Antibodix WCX	3		4.6 × 50 mm	602NP3P-4605
Antibodix WCX	3		2.1 × 50 mm	602NP3P-2105
Antibodix WCX	10		7.8 × 50 mm	602NP10-7805
Antibodix WCX	10		7.8 × 250 mm	602NP10-7825
Antibodix WCX	10		7.8 × 150 mm	602NP10-7815
Antibodix WCX	10		4.6 × 50 mm	602NP10-4605
Antibodix WCX	10		4.6 × 30 mm	602NP10-4603
Antibodix WCX	10		4.6 × 150 mm	602NP10-4615
Antibodix WCX	10		4.6 × 100 mm	602NP10-4610
Antibodix WCX	10		4 × 10 mm	602NP10-4001
Antibodix WCX	10		30 × 50 mm	602NP10-30005
Antibodix WCX	10		30 × 250 mm	602NP10-30025
Antibodix WCX	10		30 × 150 mm	602NP10-30015
Antibodix WCX	10		30 × 100 mm	602NP10-30010
Antibodix WCX	10		21.2 × 50 mm	602NP10-21205
Antibodix WCX	10		21.2 × 250 mm	602NP10-21225
Antibodix WCX	10		21.2 × 150 mm	602NP10-21215
Antibodix WCX	10		2.1 × 50 mm	602NP10-2105
Antibodix WCX	10		2.1 × 30 mm	602NP10-2103
Antibodix WCX	10		2.1 × 250 mm	602NP10-2125
Antibodix WCX	10		2.1 × 150 mm	602NP10-2115
Antibodix WCX	10		2.1 × 100 mm	602NP10-2110
Antibodix WCX	10		2 × 10 mm	602NP10-2001
Antibodix WCX	10		10 × 250 mm	602NP10-10025
Antibodix WCX	10		4.6 × 50 mm	602NP10P-4605
Antibodix WCX	10		2.1 × 50 mm	602NP10P-2105
Antibodix WCX	10		2.1 × 250 mm	602NP10P-2125
Antibodix WCX	1.7		4.6 × 50 mm	602NP2-4605
Antibodix WCX	1.7		4.6 × 30 mm	602NP2-4603
Antibodix WCX	1.7		4.6 × 100 mm	602NP2-4610
Antibodix WCX	1.7		4 × 10 mm	602NP2-4001
Antibodix WCX	1.7		2 × 10 mm	602NP2-2001
Antibodix WCX	1.7		4.6 × 50 mm	602NP2P-4605
Proteomix SCX	10		21.2 × 10 mm	401NP10-21201C

Overview Specifications Applications Method Development Troubleshooting Contact

Overview

Named phases: Antibodix WCX, Proteomix AAV-SAX, Proteomix POR Q, Proteomix POR S, Proteomix POR-Q, Proteomix POR15-HQ, Proteomix POR15-Q, Proteomix POR15-S, Proteomix SAX, Proteomix SCX, Proteomix WAX, Proteomix WCX

Separation category: Ion exchange chromatography (IEX)

Available particle sizes: 1.7, 3, 5, 10, 15, 30 µm

Available pore sizes: 1000 Å

Scientific principle

Ion exchange chromatography separates analytes through reversible electrostatic attraction between charged molecules and oppositely charged functional groups on the stationary phase. Anion exchangers retain negatively charged analytes, while cation exchangers retain positively charged analytes. Selectivity is controlled mainly by pH, buffer identity, ionic strength and gradient profile.

Strong exchangers maintain charge across a wider pH range, while weak exchangers provide charge behavior that varies with pH and may offer different selectivity. Final operating limits and cleaning conditions must be confirmed for the exact catalog phase.

What are SEPAX Ion Exchange Columns?

SEPAX ion exchange columns are designed for charge-based separation and purification of proteins, monoclonal antibodies, peptides, oligonucleotides, nucleic acids and other ionizable compounds. The catalog includes strong and weak anion-exchange and cation-exchange phases in analytical and preparative dimensions.

How do ion exchange columns work?

Retention occurs when an analyte carries a charge opposite to that of the stationary phase. Binding strength is adjusted through mobile-phase pH and ionic strength. Elution is commonly achieved by increasing salt concentration or changing pH so the electrostatic interaction becomes weaker.

Stationary-phase selection

Choose an anion exchanger for negatively charged analytes under the selected buffer conditions and a cation exchanger for positively charged analytes. Strong exchangers are often selected when a stable charge is required over a broad pH range; weak exchangers may provide useful alternate selectivity.

Applications

Typical uses include protein charge-variant analysis, monoclonal-antibody characterization, biomolecule purification, oligonucleotide separation, process development and polishing steps in biopharmaceutical workflows.

Mobile phase and method development

Use a compatible low-conductivity starting buffer at a pH that provides the desired analyte charge. Optimize pH first, then adjust buffer concentration, salt-gradient slope, flow rate, temperature and sample load. Filter samples and avoid introducing excessive salt in the injection solvent when binding is required.

Cleaning and storage

Remove salts and strongly retained material using a phase-compatible wash sequence. Re-equilibrate thoroughly after cleaning and store only in the solvent recommended for the exact phase. Avoid buffer precipitation, microbial growth and abrupt changes in solvent composition.

Troubleshooting

Poor binding may result from unsuitable pH, excessive sample conductivity or incorrect exchanger type. Broad or split peaks can arise from sample overload, injection-solvent mismatch or column contamination. Retention drift often indicates buffer-preparation differences, pH variation or incomplete equilibration.

Related products

For orthogonal biomolecule separations, review Proteomix Columns, SEC Columns, Antibodix Columns and Affinity Columns. These families can support complementary charge, size, hydrophobicity or affinity-based workflows.

Troubleshooting guidance

If recovery or resolution declines, examine sample clarification, buffer conductivity, pH accuracy, gradient slope, flow rate and possible irreversible adsorption.

Cleaning and regeneration

Remove strongly retained proteins with a compatible regeneration step, then re-equilibrate thoroughly. Avoid abrupt solvent or pH changes that could damage the bonded phase or biological ligand.

Mobile phase recommendations

Select buffers according to the interaction mode. Ion-exchange methods usually require controlled pH and salt gradients; HIC methods commonly begin at higher salt and elute by reducing salt; affinity methods require ligand-compatible loading and elution conditions.

Related SEPAX product families

For complementary selectivity or workflow steps, also review SEC Columns, Antibodix Columns and Affinity Columns. These related families can support orthogonal method development, sample cleanup, confirmation or scale-up depending on the application.

Frequently asked questions

What are SEPAX ion exchange columns used for?

They are used for charge-based separation and purification of proteins, antibodies, peptides, oligonucleotides, nucleic acids and other ionizable analytes.

Should I choose an anion exchanger or a cation exchanger?

Choose an anion exchanger when the analyte is negatively charged under the selected conditions and a cation exchanger when it is positively charged.

How are analytes eluted from an ion exchange column?

Elution is commonly achieved with an increasing salt gradient or a pH change that weakens the electrostatic interaction.

Why is my sample not binding to the column?

Common causes include unsuitable pH, high sample conductivity, the wrong exchanger type or insufficient equilibration.

Can ion exchange columns be used for monoclonal antibodies?

Yes. Ion exchange chromatography is widely used for charge-variant analysis, purification development and polishing of monoclonal antibodies.

How should ion exchange columns be cleaned?

Use a phase-compatible cleaning sequence that removes salts and strongly retained material, then re-equilibrate fully. Follow the exact product instructions.

Who supplies SEPAX ion exchange columns in India?

Akira Analytical Solutions Pvt. Ltd. supplies SEPAX products in India and supports catalog identification and column selection.

Technical support for Ion Exchange Columns

Send the application, current method and preferred dimensions to Akira Analytical Solutions Pvt. Ltd..

Request assistance