HILIC Columns
SEPAX HILIC Columns support hydrophilic interaction liquid chromatography for highly polar analytes with limited reversed-phase retention. Use the searchable catalog table below to compare the listed phases, particle sizes, pore sizes, dimensions and catalog numbers, then confirm the final configuration against the intended method and instrument requirements.
HILIC Columns specifications
| Product phase | Particle size (µm) | Pore size (Å) | Dimensions | Catalog number |
|---|---|---|---|---|
| Polar-Imidazole | 3 | 120 | 4 × 10 mm | 135333-4001C |
| Polar-Imidazole | 3 | 120 | 2 × 10 mm | 135333-2001C |
| Polar-Imidazole | 5 | 120 | 4 × 10 mm | 135335-4001C |
| Polar-Imidazole | 5 | 120 | 2 × 10 mm | 135335-2001C |
| Polar-Imidazole | 10 | 120 | 4 × 10 mm | 135339-4001C |
| Polar-Imidazole | 5 | 120 | 4 × 10 mm | 135335-4001 |
| Polar-Imidazole | 5 | 120 | 7.8 × 250 mm | 135335-7825 |
| Polar-Imidazole | 5 | 120 | 7.8 × 150 mm | 135335-7815 |
| Polar-Imidazole | 5 | 120 | 4.6 × 50 mm | 135335-4605 |
| Polar-Imidazole | 5 | 120 | 4.6 × 30 mm | 135335-4603 |
| Polar-Imidazole | 5 | 120 | 4.6 × 250 mm | 135335-4625 |
| Polar-Imidazole | 5 | 120 | 4.6 × 150 mm | 135335-4615 |
| Polar-Imidazole | 5 | 120 | 4.6 × 100 mm | 135335-4610 |
| Polar-Imidazole | 5 | 120 | 30 × 50 mm | 135335-30005 |
| Polar-Imidazole | 5 | 120 | 30 × 250 mm | 135335-30025 |
| Polar-Imidazole | 5 | 120 | 30 × 150 mm | 135335-30015 |
| Polar-Imidazole | 5 | 120 | 30 × 100 mm | 135335-30010 |
| Polar-Imidazole | 5 | 120 | 21.2 × 50 mm | 135335-21205 |
| Polar-Imidazole | 5 | 120 | 21.2 × 250 mm | 135335-21225 |
| Polar-Imidazole | 5 | 120 | 21.2 × 150 mm | 135335-21215 |
| Polar-Imidazole | 5 | 120 | 21.2 × 100 mm | 135335-21210 |
| Polar-Imidazole | 5 | 120 | 2.1 × 50 mm | 135335-2105 |
| Polar-Imidazole | 5 | 120 | 2.1 × 30 mm | 135335-2103 |
| Polar-Imidazole | 5 | 120 | 2.1 × 250 mm | 135335-2125 |
| Polar-Imidazole | 5 | 120 | 2.1 × 150 mm | 135335-2115 |
| Polar-Imidazole | 5 | 120 | 2.1 × 100 mm | 135335-2110 |
| Polar-Imidazole | 5 | 120 | 2 × 10 mm | 135335-2001 |
| Polar-Imidazole | 5 | 120 | 10 × 250 mm | 135335-10025 |
| Polar-Imidazole | 5 | 120 | 10 × 150 mm | 135335-10015 |
| Polar-Imidazole | 3 | 120 | 4 × 10 mm | 135333-4001 |
| Polar-Imidazole | 3 | 120 | 2.1 × 50 mm | 135333-2105 |
| Polar-Imidazole | 3 | 120 | 2.1 × 30 mm | 135333-2103 |
| Polar-Imidazole | 3 | 120 | 2.1 × 250 mm | 135333-2125 |
| Polar-Imidazole | 3 | 120 | 2.1 × 150 mm | 135333-2115 |
| Polar-Imidazole | 3 | 120 | 2.1 × 100 mm | 135333-2110 |
| Polar-Imidazole | 3 | 120 | 2 × 10 mm | 135333-2001 |
| Polar-Imidazole | 3 | 120 | 4.6 × 50 mm | 135333-4605 |
| Polar-Imidazole | 3 | 120 | 4.6 × 30 mm | 135333-4603 |
| Polar-Imidazole | 3 | 120 | 4.6 × 250 mm | 135333-4625 |
| Polar-Imidazole | 3 | 120 | 4.6 × 150 mm | 135333-4615 |
| Polar-Imidazole | 3 | 120 | 4.6 × 100 mm | 135333-4610 |
| Polar-Imidazole | 10 | 120 | 7.8 × 250 mm | 135339-7825 |
| Polar-Imidazole | 10 | 120 | 4 × 10 mm | 135339-4001 |
| Polar-Imidazole | 10 | 120 | 30 × 50 mm | 135339-30005 |
| Polar-Imidazole | 10 | 120 | 30 × 250 mm | 135339-30025 |
| Polar-Imidazole | 10 | 120 | 30 × 150 mm | 135339-30015 |
| Polar-Imidazole | 10 | 120 | 30 × 100 mm | 135339-30010 |
| Polar-Imidazole | 10 | 120 | 21.2 × 50 mm | 135339-21205 |
| Polar-Imidazole | 10 | 120 | 21.2 × 250 mm | 135339-21225 |
| Polar-Imidazole | 10 | 120 | 21.2 × 150 mm | 135339-21215 |
| Polar-Imidazole | 10 | 120 | 21.2 × 100 mm | 135339-21210 |
| Polar-Imidazole | 10 | 120 | 10 × 250 mm | 135339-10025 |
| Polar-Imidazole | 10 | 120 | 10 × 150 mm | 135339-10015 |
| Polar-Imidazole | 10 | 120 | 10 × 100 mm | 135339-10010 |
| Polar-Imidazole | 1.8 | 120 | 4.6 × 50 mm | 135331-4605 |
| Polar-Imidazole | 1.8 | 120 | 4.6 × 30 mm | 135331-4603 |
| Polar-Imidazole | 1.8 | 120 | 4.6 × 150 mm | 135331-4615 |
| Polar-Imidazole | 1.8 | 120 | 4.6 × 100 mm | 135331-4610 |
| Polar-Imidazole | 1.8 | 120 | 2.1 × 50 mm | 135331-2105 |
| Polar-Imidazole | 1.8 | 120 | 2.1 × 30 mm | 135331-2103 |
| Polar-Imidazole | 1.8 | 120 | 2.1 × 150 mm | 135331-2115 |
| Polar-Imidazole | 1.8 | 120 | 2.1 × 100 mm | 135331-2110 |
Overview
Named phases: Polar-Imidazole
Separation categories: HILIC
Available particle sizes: 1.8, 3, 5, 10 µm
Available pore sizes: 120 Å
Scientific principle
HILIC retains polar analytes on a polar stationary phase under mobile phases containing a high proportion of organic solvent and an aqueous component.
For detailed surface chemistry and packing guidance, contact technical support.
What are SEPAX HILIC Columns?
SEPAX HILIC Columns are catalog-listed products for hydrophilic interaction liquid chromatography. The family is intended for highly polar analytes with limited reversed-phase retention, with available configurations shown in the specification table on this page.
How do HILIC Columns work?
The separation is governed by partitioning into a water-enriched layer at the polar stationary-phase surface. Selectivity also depends on the exact phase, mobile phase, sample properties, temperature, flow rate and column dimensions.
How to select a configuration
Start with the required separation mechanism and named phase. Then compare particle size, pore size where listed, internal diameter, length and catalog number. Match the configuration to system pressure capability, sample load and the scale of the method.
Applications
This family is relevant to glycan, polar metabolite, sugar and hydrophilic impurity analysis. Suitability must be confirmed experimentally with the actual sample and method conditions.
Method development
Begin with a documented method or a chemically reasonable starting condition for the selected phase. Control sample solvent strength, buffer compatibility, pH, ionic strength, gradient profile, temperature and injection volume. Change one variable at a time and record system-suitability results.
Column care and storage
Filter samples and mobile phases, prevent buffer precipitation, avoid sudden solvent incompatibility and stay within the product-specific operating limits supplied with the column. After use, remove strongly retained material with a compatible wash and store the column only in a suitable solvent for that exact phase.
Replacement and method transfer
Do not select a replacement by dimensions alone. Compare separation mechanism, stationary-phase type, particle size, pore size where applicable, column geometry and method conditions. Verify equivalence through retention, resolution, efficiency, tailing and system-suitability testing.
For catalog identification and application support, contact SEPAX technical support in India.
Troubleshooting guidance
Retention drift and irregular peak shape frequently result from insufficient equilibration, sample solvent mismatch, variable water content or partially precipitated buffer.
Cleaning and regeneration
Flush buffers before storage and avoid salt precipitation in high-organic mobile phase. Re-equilibrate with multiple column volumes because HILIC water-layer formation can be slower than reversed-phase equilibration.
Mobile phase recommendations
HILIC methods usually begin with a high proportion of acetonitrile and a smaller aqueous buffer fraction. Keep buffer concentration and water content reproducible because small changes can strongly affect retention.
Related SEPAX product families
For complementary selectivity or workflow steps, also review HP Columns, Carbomix Sugar Columns and GP Columns. These related families can support orthogonal method development, sample cleanup, confirmation or scale-up depending on the application.
Frequently asked questions
What are HILIC Columns?
HILIC Columns are SEPAX catalog products used for hydrophilic interaction liquid chromatography. The listed configurations can be compared by phase, particle size, pore size where applicable, dimensions and catalog number.
How do HILIC Columns work?
They separate analytes through partitioning into a water-enriched layer at the polar stationary-phase surface. The exact behavior depends on the selected phase and method conditions.
Which HILIC Columns should I choose?
Choose first by separation mechanism and named phase, then by particle size, pore size where listed, internal diameter, length, sample load and system pressure capability.
Does this page show the catalog configurations for HILIC Columns?
Yes. The searchable specification table presents the configurations included in the website catalog data for this family. Use the catalog number when requesting confirmation or a quotation.
What applications are suitable for HILIC Columns?
This family is relevant to glycan, polar metabolite, sugar and hydrophilic impurity analysis. Final suitability depends on the analyte, sample matrix and method requirements.
Who supplies HILIC Columns in India?
Akira Analytical Solutions Pvt. Ltd. supplies SEPAX products in India and assists with catalog identification, column selection and technical enquiries.
Technical support for HILIC Columns
Send the application, current method and preferred dimensions to Akira Analytical Solutions Pvt. Ltd..