GPC Columns
SEPAX GPC Columns support gel permeation chromatography for polymers and macromolecules. Use the searchable catalog table below to compare the listed phases, particle sizes, pore sizes, dimensions and catalog numbers, then confirm the final configuration against the intended method and instrument requirements.
GPC Columns specifications
| Product phase | Particle size (µm) | Pore size (Å) | Dimensions | Catalog number |
|---|---|---|---|---|
| Mono GPC | 5 | 1000 | 7.8 × 50 mm | 231000-7805 |
| Mono GPC | 5 | 100 | 7.8 × 50 mm | 230100-7805 |
| Mono GPC | 5 | 100-1000 | 7.8 × 50 mm | 231135-7805 |
| Mono GPC | 5 | 100-1000 | 7.8 × 300 mm | 231135-7830 |
| Mono GPC | 5 | 100-1000 | 4.6 × 300 mm | 231135-4630 |
| Mono GPC | 5 | 500 | 7.8 × 50 mm | 230500-7805 |
| Mono GPC | 5 | 500 | 7.8 × 300 mm | 230500-7830 |
| Mono GPC | 5 | 500 | 4.6 × 300 mm | 230500-4630 |
| Mono GPC | 10 | 500 | 7.8 × 300 mm | 240500-7830 |
| Mono GPC | 10 | 500 | 4.6 × 300 mm | 240500-4630 |
| Mono GPC | 10 | 500 | 21.2 × 300 mm | 240500-21230 |
| Mono GPC | 5 | 300 | 7.8 × 50 mm | 230300-7805 |
| Mono GPC | 5 | 300 | 7.8 × 300 mm | 230300-7830 |
| Mono GPC | 5 | 300 | 4.6 × 300 mm | 230300-4630 |
| Mono GPC | 10 | 300 | 7.8 × 300 mm | 240300-7830 |
| Mono GPC | 10 | 300 | 4.6 × 300 mm | 240300-4630 |
| Mono GPC | 10 | 100-1000 | 7.8 × 300 mm | 241135-7830 |
| Mono GPC | 10 | 100-1000 | 4.6 × 300 mm | 241135-4630 |
| Mono GPC | 5 | 1000 | 7.8 × 300 mm | 231000-7830 |
| Mono GPC | 5 | 1000 | 4.6 × 300 mm | 231000-4630 |
| Mono GPC | 10 | 1000 | 7.8 × 300 mm | 241000-7830 |
| Mono GPC | 10 | 1000 | 4.6 × 300 mm | 241000-4630 |
| Mono GPC | 10 | 100 | 4.6 × 50 mm | 240100-4605 |
| Mono GPC | 5 | 100 | 7.8 × 300 mm | 230100-7830 |
| Mono GPC | 5 | 100 | 4.6 × 300 mm | 230100-4630 |
| Mono GPC | 10 | 100 | 7.8 × 300 mm | 240100-7830 |
| Mono GPC | 10 | 100 | 4.6 × 300 mm | 240100-4630 |
| Mono GPC | 10 | 100 | 21.2 × 300 mm | 240100-21230 |
| Mono GPC | 10 | 100 | 21.1 × 250 mm | 240100-21225 |
| Mono GPC | 5 | 100 | 21.2 × 10 mm | 230100-21201C |
| Mono GPC | 5 | 100 | 21.2 × 10 mm | 230100-21201 |
Overview
Named phases: Mono GPC
Separation categories: SEC
Available particle sizes: 5, 10 µm
Available pore sizes: 100, 100-1000, 300, 500, 1000 Å
Scientific principle
Size exclusion chromatography separates molecules primarily by their access to pores within the packing material. Larger species generally access less pore volume and elute earlier than smaller species.
For detailed surface chemistry and packing guidance, contact technical support.
What are SEPAX GPC Columns?
SEPAX GPC Columns are catalog-listed products for gel permeation chromatography. The family is intended for polymers and macromolecules, with available configurations shown in the specification table on this page.
How do GPC Columns work?
The separation is governed by molecular size and access to the pore network. Selectivity also depends on the exact phase, mobile phase, sample properties, temperature, flow rate and column dimensions.
How to select a configuration
Start with the required separation mechanism and named phase. Then compare particle size, pore size where listed, internal diameter, length and catalog number. Match the configuration to system pressure capability, sample load and the scale of the method.
Applications
This family is relevant to molecular-weight distribution and polymer characterization. Suitability must be confirmed experimentally with the actual sample and method conditions.
Method development
Begin with a documented method or a chemically reasonable starting condition for the selected phase. Control sample solvent strength, buffer compatibility, pH, ionic strength, gradient profile, temperature and injection volume. Change one variable at a time and record system-suitability results.
Column care and storage
Filter samples and mobile phases, prevent buffer precipitation, avoid sudden solvent incompatibility and stay within the product-specific operating limits supplied with the column. After use, remove strongly retained material with a compatible wash and store the column only in a suitable solvent for that exact phase.
Replacement and method transfer
Do not select a replacement by dimensions alone. Compare separation mechanism, stationary-phase type, particle size, pore size where applicable, column geometry and method conditions. Verify equivalence through retention, resolution, efficiency, tailing and system-suitability testing.
For catalog identification and application support, contact SEPAX technical support in India.
Troubleshooting guidance
Unstable baselines, pressure changes and distorted distributions may be caused by incomplete dissolution, particulates, solvent mismatch, air or temperature variation.
Cleaning and regeneration
Flush with clean compatible solvent and avoid rapid switching between solvents with very different polarity or viscosity. Store the column filled with an approved solvent.
Mobile phase recommendations
Use fully compatible organic solvents of consistent grade and viscosity. Dissolve and filter polymer samples completely, and match sample solvent closely to the mobile phase.
Related SEPAX product families
For complementary selectivity or workflow steps, also review SEC Columns, HP Columns and Accessories. These related families can support orthogonal method development, sample cleanup, confirmation or scale-up depending on the application.
Frequently asked questions
What are GPC Columns?
GPC Columns are SEPAX catalog products used for gel permeation chromatography. The listed configurations can be compared by phase, particle size, pore size where applicable, dimensions and catalog number.
How do GPC Columns work?
They separate analytes through molecular size and access to the pore network. The exact behavior depends on the selected phase and method conditions.
Which GPC Columns should I choose?
Choose first by separation mechanism and named phase, then by particle size, pore size where listed, internal diameter, length, sample load and system pressure capability.
Does this page show the catalog configurations for GPC Columns?
Yes. The searchable specification table presents the configurations included in the website catalog data for this family. Use the catalog number when requesting confirmation or a quotation.
What applications are suitable for GPC Columns?
This family is relevant to molecular-weight distribution and polymer characterization. Final suitability depends on the analyte, sample matrix and method requirements.
Who supplies GPC Columns in India?
Akira Analytical Solutions Pvt. Ltd. supplies SEPAX products in India and assists with catalog identification, column selection and technical enquiries.
Technical support for GPC Columns
Send the application, current method and preferred dimensions to Akira Analytical Solutions Pvt. Ltd..